Sample preperation - Derivatisation - Hydrolysis and derivatization for monosugar composition analysis

5 important questions on Sample preperation - Derivatisation - Hydrolysis and derivatization for monosugar composition analysis

What happens if your hydrolysis conditions are to mild?

  • Not a representative composition
  • Insoluble fibres, uronic acids and cellulose difficult to hydryose complete
  • No generic method for both soluble and insoluble polysaccharides.

What happens if your hydrolysis conditions are to harsh?

Sensitive sugars are lost more readily

How do you hydrolyse fibrous materials?

  • 12 M H2SO4 for 1 hour at 30 degrees
  • 1M H2SO4, 3H at 100 degrees.
  • Higher grades + faster learning
  • Never study anything twice
  • 100% sure, 100% understanding
Discover Sailabus

How do you hydrolyse uronic acid containing materials (pectin)?

2M HCl in methanol, 16h at 80 degrees

How can you analyse sugar composition with GC?

By first a reduction step (bound O become a OH) and then do a acetylation step so the sugar becomes volatile.

The question on the page originate from the summary of the following study material:

  • A unique study and practice tool
  • Never study anything twice again
  • Get the grades you hope for
  • 100% sure, 100% understanding
Remember faster, study better. Scientifically proven.
Trustpilot Logo